Regulation of intracellular pH, gas channels. We have three major research areas see below. Our three projects interact philosophically and technically. The lungs, controlled by brainstem neurons, regulate pH o by regulating [CO 2 ] o.
Ph.Done! … but still not a Dr.
Western blot or western blot? - General Biology Discussion
The western blot sometimes called the protein immunoblot , or western blotting , is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. Western blot technique uses three elements to achieve its task of separating a specific protein from a complex: separation by size, transfer of protein to a solid support, and marking target protein using a primary and secondary antibody to visualize. A synthetic or animal-derived antibody known as the primary antibody is created that recognizes and binds to a specific target protein. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognizes and binds to the primary antibody. The secondary antibody is visualized through various methods such as staining , immunofluorescence , and radioactivity, allowing indirect detection of the specific target protein.
The thesis was found to be scientifically valid, and a decision was made to allow Nitin Aggarwal to retain his terminal degree, but with notations on the findings placed in his academic record. Aggarwal is now working at Bristol-Myers Squibb. Update, p. For the paper by Nitin T.
Hi all, A rather odd question but I'm writing my PhD thesis and I'd like to know whether you spell Western blot with a capital 'W' or a small case 'w' when it's in the middle of a sentence? I've seen it spelt both with 'W' and 'w' so is it just preference? Thanks Sxxx.